Third-Generation Nanopore Sequencing Fundamentals and Bioinformatics

Third-generation sequencing has transformed genomics by enabling direct, real-time analysis of long DNA and RNA fragments. Understanding Nanopore sequencing opens modern capabilities for genome assembly, structural variant detection, and metagenomic profiling. This written course provides a comprehensive foundation in the technology, guiding you from fundamental biological mechanics to command-line bioinformatics pipelines. What you'll learn: - Understand core principles of nanopore sensing and biological strand sequencing - Explore available hardware platforms, flow cell architectures, and library preparation protocols - Master modern basecalling models and raw signal processing concepts for high-accuracy reads - Analyze long-read datasets for genome assembly, read alignment, and variant calling - Apply quality control, read filtering, and adaptive sampling strategies to downstream analysis - Practice bioinformatics workflows using standard command-line software for long-read data The course begins with foundational concepts, key terminology, and physical principles before walking through practical bioinformatics processing steps and data evaluation. Designed specifically for beginners, researchers, and students in biology or computer science, this text-based guide requires no prior experience with sequencing tools. Start reading today to build practical skills in long-read genomics.